Speaker
Description
In the biomedical field, several applications for the High Power Laser Systems are proposed. Delivering a high dose in a very short time and understanding the biomolecular effects of radiation is a challenge with implications for clinical translation. Biological systems are impacted differently by various radiation dose-rates. Finding new ways to detect dose-rate effects as early as possible is mandatory.
We propose a protocol for detecting via nuclear magnetic resonance spectroscopy (NMR) differences in the metabolic profiles of cells irradiated using secondary radiation generated at the interaction of the High Power Laser System with a gas target at ELI-NP. We have developed an NMR method for radiation effects in cells. The irradiation setup we proposed uses secondary radiation (electrons) stemming from the interaction chamber of 1 PW laser, operating in pulses delivered onto a gas target. Variations in metabolite concentrations between control and irradiated samples for BV-2 Microglia cells (non-tumoral cells) were measured using high-resolution Nuclear Magnetic Resonance spectroscopy.
We show that Magnetic Resonance biomarkers can be used for timely detection of radiation dose-rate effects up to the Gy/ns regime.
